High Affinity and Low Affinity Binding of Ca++ by Rat Liver Mitochondria
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چکیده
منابع مشابه
Studies on the nature of the high-affinity trialkyltin binding site of rat liver mitochondria.
1. The proteolipid fraction isolated from rat liver mitochondria pretreated with [3H]triphenyltin chloride is enriched in triphenyltin compared with the original mitochondria. 2. Part of this [3H]triphenyltin is eluted with a protein of Mr 5000-6000 on Sephadex LH20 chromatography. 2. Mössbauer spectra of the proteolipid fraction treated with 119Sn-enriched triethyltin chloride show a doublet w...
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1. alpha-Cyano-4-hydroxycinnamate was coupled to Sepharose CL-4B activated with 1,2:3,4-bisepoxybutane. 2. The low-Km rat liver mitochondrial aldehyde dehydrogenase was specifically bound to this affinity medium, and could subsequently be eluted with alpha-cyano-4-hydroxycinnamate. 3. The enzyme purified in this manner had a subunit molecular mass of 55 kDa and a pI of approx. 6.5. A minor comp...
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When binding of 125I-alpha bungarotoxin (125I-alpha BTX) to hypothalamic membranes is observed over a wide range of concentrations, 3 binding sites can be identified, with estimated equilibrium dissociation constants (Kds) of 4.1 X 10(-11) M, 6.2 X 10(-10) M, and 9.1 X 10(-7) M for high-, low-, and very-low-affinity interactions, respectively. The densities of the high- and low-affinity sites w...
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Although DNA is often bent in vivo, it is unclear how DNA-bending forces modulate DNAprotein binding affinity. Here, we report how a range of DNA-bending forces modulates the binding of the Integration Host Factor (IHF) protein to various DNAs. Using solution fluorimetry and electrophoretic mobility shift assays, we measured the affinity of IHF for DNAs with different bending forces and sequenc...
متن کاملHigh-affinity folate binding in human liver membranes.
High-affinity binding of 3H-folate in Triton X-100 solubilized membranes of human liver displayed characteristics, e.g. apparent positive cooperativity, which are typical of specific folate binding. Ultrogel AcA 44 chromatography of solubilized membranes saturated with 3H-folate revealed a major peak of 100 kDa and a minor peak of 25 kDa. The 100 kDa peak could represent a hydrophobic membrane ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)94396-6